ABSTRACT

In the Molecular Characterization of an Unknown P-element Insertion in Drosophila melanogaster , a plasmid rescue was performed in order to identify the location of the target gene in the fly genome. Based on phenotypic evidence, this gene causes sterility in homozygous female flies. The purpose of this team project was to clone the P-element and the gene in which it was contained. After DNA isolation and cleavage, the DNA fragments were ligated to form circular plasmids and transformed into E. coli bacteria to be cloned. After incubation for 24 hours on ampicilin-containing agar plates, no bacterial colony growth was observed.