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2006 Team 8

IDENTIFICATION AND CHARACTERIZATION OF ENTEROCOCCUS SPP. IN LOCAL SURFACE WATERS

Neha Bansal, Laura Decker, Kevin Huang, Ashwinee Ragam, Angela Sekerke, Reema Shah, Sarah Song, Pallavi Yerramilli, Eddie Zhang, Jeremy Zornow

Advisor: Dr. June Middleton
Assistant: Alex Kohl

ABSTRACT

In order to identify and characterize Enterococcus spp. , we isolated bacteria from surface water samples taken from four sites in Morris County, NJ. We then performed various biochemical tests to identify the isolates by species, as well as determine their antibiotic resistance and virulence. Through the use of bacterial count data, this study indicated that none of the four local water sources were suitable for bathing or drinking water, according to federal safe water standards. Naturally found in animal digestive tracts, Enterococcus spp. is often utilized as fecal indicators in surface waters. In addition, we concluded that many common antibiotics, such as penicillin, are no longer effective in combating Enterococcus spp. Due to the recent increase in the number of nosocomial pathogens, many studies have shifted their focus to two of the more reoccurring and pathogenic species - Enterococcus faecalis and Enterococcus faecium . These two Enterococcus species are known for their resilience and high level of antimicrobial resistance. E. faecium was isolated from all four of the study sites; however, E. faecalis was only present at Loantaka Brook and Drew Pond. These two particular species of Enterococcus are responsible for a variety of diseases, including bacteriemia, endocarditis, urinary tract infections, and wound infections [1].

 
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